Vascular-associated lymphoid tissue in swine (Sus scrofa)

Ingeborg M. Langohr, Harm HogenEsch, Gregory W. Stevenson, Michael Sturek

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Focal accumulations of mononuclear cells in the arterial wall of healthy humans at predilection sites for atherosclerotic lesions have been described as 'vascular-associated lymphoid tissue' (VALT). Here we investigated whether pigs (Sus scrofa), a commonly used animal model for studying cardiovascular disease, have VALT. Samples of major arteries were collected from 10 conventional crossbred pigs (age, 2 to 24 mo) and processed for routine light microscopy, immunohistochemistry, and immunofluorescence. Single or small aggregates of mononuclear cells were noted in the intima and occasionally the inner portion of the tunica media and adventitia at branching sites. The infiltrating cells were primarily CD3+CD4+ T cells, with some macrophages. No CD8+ T cells were present. Infiltrating leukocytes and overlying endothelial cells frequently expressed major histocompatibility class II molecules. Two Ossabaw pigs on low-fat diet had similar leukocytic aggregates at locations where animals of the same breed but fed a high-fat and high-cholesterol diet developed atherosclerotic lesions. Further, the densities of CD3+ T lymphocytes and in these areas were decreased in 2 sedentary and 2 exercised Ossabaw pigs on an atherogenic diet compared with conventional crossbred and Ossabaw pigs on a normal diet. This study shows that focal aggregates of lymphocytes occur in the vasculature of pigs at locations predisposed to development of atherosclerotic lesions. These cellular aggregates are similar to the structures described as VALT in human arteries and reinforce the value of the pig as a model for the study of human cardiovascular disease.

Original languageEnglish
Pages (from-to)168-173
Number of pages6
JournalComparative Medicine
Volume58
Issue number2
StatePublished - Apr 2008

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Sus scrofa
Lymphoid Tissue
Nutrition
blood vessels
Blood Vessels
T-cells
Swine
Tissue
swine
cell aggregates
lesions (animal)
Animals
Fats
T-lymphocytes
Lymphocytes
Macrophages
T-Lymphocytes
Endothelial cells
arteries
cardiovascular diseases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Vascular-associated lymphoid tissue in swine (Sus scrofa). / Langohr, Ingeborg M.; HogenEsch, Harm; Stevenson, Gregory W.; Sturek, Michael.

In: Comparative Medicine, Vol. 58, No. 2, 04.2008, p. 168-173.

Research output: Contribution to journalArticle

Langohr, Ingeborg M. ; HogenEsch, Harm ; Stevenson, Gregory W. ; Sturek, Michael. / Vascular-associated lymphoid tissue in swine (Sus scrofa). In: Comparative Medicine. 2008 ; Vol. 58, No. 2. pp. 168-173.
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