Vascular damage by ultrasound-activated microbubble induced vesselinvagination

Hong Chen, Andrew A. Brayman, Thomas J. Matula, Andrew P. Evan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Vascular bioeffects produced by ultrasound contrast agent microbubbles areprimarily manifested as damage to microvessels. The objective of this work is todirectly observe the transient dynamics of bubble-vessel interactions andcorrelate the observed interactions with associated vascular damage.Microbubbles were perfused into microvessels in ex vivo rat mesenteries and thenexcited by a single 2 s long ultrasound pulse at 1 MHz. Meanwhile, 14high-speed photomicrographic images were acquired using 50 ns shutter speeds.The targeted region was then examined by histology and transmission electronmicroscopy (TEM). Image registration was used to identify the specific vesselsthat the corresponding high-speed images were captured. The recorded high-speedimages revealed that bubble-vessel interactions caused vessel wall distention(motion outward against the surrounding tissue) and invagination (motion inwardtoward the lumen). Invagination exceeding distention was observed in 60 out of70 cases. Significant vessel invagination was correlated with vascular damagethat was characterized by a separation of the endothelium from the surroundingtissue as revealed by both the histology and TEM analyses. The separation of theendothelium from the surrounding tissue is consistent with damage caused bytensile stresses at the vessel walls that lead to vessel invagination. Thissuggests that invagination may be an important mechanism by which microbubblescause vascular damage.

Original languageEnglish (US)
Title of host publication2010 IEEE International Ultrasonics Symposium, IUS 2010
Pages678-681
Number of pages4
DOIs
StatePublished - Dec 1 2010
Event2010 IEEE International Ultrasonics Symposium, IUS 2010 - San Diego, CA, United States
Duration: Oct 11 2010Oct 14 2010

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117

Other

Other2010 IEEE International Ultrasonics Symposium, IUS 2010
CountryUnited States
CitySan Diego, CA
Period10/11/1010/14/10

Keywords

  • microbubbles
  • microvessels
  • vascular damage
  • vessel invagiantion

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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    Chen, H., Brayman, A. A., Matula, T. J., & Evan, A. P. (2010). Vascular damage by ultrasound-activated microbubble induced vesselinvagination. In 2010 IEEE International Ultrasonics Symposium, IUS 2010 (pp. 678-681). [5935994] (Proceedings - IEEE Ultrasonics Symposium). https://doi.org/10.1109/ULTSYM.2010.5935994