Vascular endothelial growth factor (VEGF) stimulates monocyte migration through endothelial monolayers via increased integrin expression

Matthias Heil, Matthias Clauss, Keisuke Suzuki, Ivo R. Buschmann, Antje Willuweit, Svaantje Fischer, Wolfgang Schaper

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Monocytes play an important role in collateral vessel formation (arteriogenesis) by attaching to activated endothelium and by invading the walls of innate collateral vessels where they produce growth factors. Previous studies have demonstrated that this process can be promoted by several chemokines and growth factors. In this study we examined the interaction between monocytes and endothelium under stimulation of the angiogenic agent vascular endothelial growth factor (VEGF). We report here the novel finding that VEGF stimulates the expression of the α(L)-, α(M)- and β2-integrin monomers. In functional assays and by using neutralizing antibodies it was shown that VEGF stimulates adhesion of monocytes to human umbilical vein endothelial cells (HUVEC), and increased transmigration through endothelial monolayers is dependent on interaction of monocyte β2-integrins with its endothelial counter ligand ICAM-1. Based on these in vitro data we hypothesize that the positive effect of VEGF on arteriogenesis may involve monocyte activation.

Original languageEnglish (US)
Pages (from-to)850-857
Number of pages8
JournalEuropean Journal of Cell Biology
Volume79
Issue number11
StatePublished - 2000
Externally publishedYes

Fingerprint

Integrins
Vascular Endothelial Growth Factor A
Monocytes
Endothelium
Intercellular Signaling Peptides and Proteins
Transendothelial and Transepithelial Migration
Human Umbilical Vein Endothelial Cells
Intercellular Adhesion Molecule-1
Neutralizing Antibodies
Chemokines
Ligands

Keywords

  • Arteriogenesis (collateral artery growth)
  • Cell adhesion molecules
  • Integrins
  • Monocytes
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Vascular endothelial growth factor (VEGF) stimulates monocyte migration through endothelial monolayers via increased integrin expression. / Heil, Matthias; Clauss, Matthias; Suzuki, Keisuke; Buschmann, Ivo R.; Willuweit, Antje; Fischer, Svaantje; Schaper, Wolfgang.

In: European Journal of Cell Biology, Vol. 79, No. 11, 2000, p. 850-857.

Research output: Contribution to journalArticle

Heil, Matthias ; Clauss, Matthias ; Suzuki, Keisuke ; Buschmann, Ivo R. ; Willuweit, Antje ; Fischer, Svaantje ; Schaper, Wolfgang. / Vascular endothelial growth factor (VEGF) stimulates monocyte migration through endothelial monolayers via increased integrin expression. In: European Journal of Cell Biology. 2000 ; Vol. 79, No. 11. pp. 850-857.
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AU - Fischer, Svaantje

AU - Schaper, Wolfgang

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