Vascular lesions in pigs experimentally infected with porcine circovirus type 2 serogroup B

I. M. Langohr, G. W. Stevenson, E. A. Nelson, S. D. Lenz, Harm HogenEsch, H. Wei, R. M. Pogranichniy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Vasculitis is a hallmark lesion of the severe form of systemic porcine circovirus-associated disease (PCVAD). In 2 experimental studies with porcine circovirus type 2 serogroup b (PCV2b), 2 pigs developed fatal PCVAD with acute vasculitis, and 5 related pigs developed chronic lymphohistiocytic and plasmacytic peri- and endarteritis. Five of these pigs (1 with acute vasculitis and 4 with chronic vasculitis) had also been inoculated with bovine viral diarrhea virus type 1 (BVDV1) or BVDV1-like virus. Vascular lesions were similar, independent of whether pigs had been inoculated singly with PCV2b or dually with PCV2b and BVDV1 or BVDV1-like virus. The acute vasculitis was accompanied by marked pulmonary and mesenteric edema and pleural effusion. In situ hybridization demonstrated abundant intracytoplasmic porcine circovirus type 2 (PCV2) nucleic acid in endothelial, smooth muscle-like, and inflammatory cells within and around affected arteries. The pigs with lymphohistiocytic and plasmacytic vasculitis had lesions of systemic PCVAD, including multisystemic lymphoplasmacytic and histiocytic or granulomatous inflammation. PCV2 nucleic acid was detected in renal tubule epithelial cells, mononuclear inflammatory cells, and rare endothelial cells in noninflamed vessels in multiple tissues of these animals. The 2 pigs with acute vasculitis had no PCV2-specific antibodies (or a low titer of), whereas the pigs with lymphohistiocytic and plasmacytic vasculitis developed high antibody titers against this virus. These observations suggest that (1) acute vasculitis observed in the current studies is directly caused by PCV2b, (2) chronic vasculitis may in part be mediated by the subsequent immune response, and (3) host factors and viral strain may both contribute to vasculitis in animals infected with PCV2b.

Original languageEnglish (US)
Pages (from-to)140-147
Number of pages8
JournalVeterinary Pathology
Volume47
Issue number1
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Circovirus
vasculitis
Porcine circovirus-2
Vasculitis
blood vessels
lesions (animal)
Blood Vessels
serotypes
Swine
swine
Bovine viral diarrhea virus 1
Porcine circovirus
Nucleic Acids
nucleic acids
Serogroup
Endarteritis
Viruses
viruses
renal tubules
antibodies

Keywords

  • Porcine circovirus type 2
  • Porcine circovirus type 2-associated disease
  • Swine
  • Vascular lesions
  • Viral infection

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Langohr, I. M., Stevenson, G. W., Nelson, E. A., Lenz, S. D., HogenEsch, H., Wei, H., & Pogranichniy, R. M. (2010). Vascular lesions in pigs experimentally infected with porcine circovirus type 2 serogroup B. Veterinary Pathology, 47(1), 140-147. https://doi.org/10.1177/0300985809352793

Vascular lesions in pigs experimentally infected with porcine circovirus type 2 serogroup B. / Langohr, I. M.; Stevenson, G. W.; Nelson, E. A.; Lenz, S. D.; HogenEsch, Harm; Wei, H.; Pogranichniy, R. M.

In: Veterinary Pathology, Vol. 47, No. 1, 2010, p. 140-147.

Research output: Contribution to journalArticle

Langohr, IM, Stevenson, GW, Nelson, EA, Lenz, SD, HogenEsch, H, Wei, H & Pogranichniy, RM 2010, 'Vascular lesions in pigs experimentally infected with porcine circovirus type 2 serogroup B', Veterinary Pathology, vol. 47, no. 1, pp. 140-147. https://doi.org/10.1177/0300985809352793
Langohr, I. M. ; Stevenson, G. W. ; Nelson, E. A. ; Lenz, S. D. ; HogenEsch, Harm ; Wei, H. ; Pogranichniy, R. M. / Vascular lesions in pigs experimentally infected with porcine circovirus type 2 serogroup B. In: Veterinary Pathology. 2010 ; Vol. 47, No. 1. pp. 140-147.
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