Vasculature in pre-blastema and nerve-dependent blastema stages of regenerating forelimbs of the adult newt, Notophthalmus viridescens

Mona A.E. Rageh, Luciara Mendenhall, Essam E.A. Moussad, Sarah E. Abbey, Anthony L. Mescher, Roy A. Tassava

Research output: Contribution to journalArticle

31 Scopus citations


Immunocytochemistry utilizing a monoclonal antibody (BV1; blood vessel 1) highly reactive to the vasculature of the adult newt showed that a developing vasculature was present during early, pre-blastema, and early-bud blastema stages of forelimb regeneration in this species. Infusion of Prussian Blue and DiI into the brachial artery further delineated the intactness of this early vasculature. Finally, macroscopic observations of vascular flow underneath the apical epithelial cap (AEC) and microsurgical removal of the AEC and observation of subsequent bleeding buttressed the conclusion that an intact vasculature exists during early nerve-dependent stages of newt forelimb regeneration. The results suggest that this process of neovascular formation is angiogenesis, i.e., the formation of new vessels from pre-existing vessels in the stump. Furthermore, angiogenesis is an ongoing process initiated early after amputation. Blastema cells and the AEC are likely sources of factors that stimulate neovascularization.

Original languageEnglish (US)
Pages (from-to)255-266
Number of pages12
JournalJournal of Experimental Zoology
Issue number3
StatePublished - Feb 15 2002


ASJC Scopus subject areas

  • Animal Science and Zoology

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