VEGF-A regulates the expression of VEGF-C in human retinal pigment epithelial cells

B. Zhao, A. Ma, J. Cai, Mike Boulton

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Aim: To determine the expression and regulation of vascular endothelial growth factor C (VEGF-C), and its receptor VEGFR-3, in human retinal pigment epithelial (RPE) cells and to consider their angiogenic role in choroidal neovascularisation (CNV). Method: The expression of VEGF-C and VEGFR-3 in cultured human RPE was confirmed by immunostaining, PCR, western blotting, and ELISA. Cultured RPE cells were exposed to VEGF-A and glucose and VEGF-C and VEGFR-3 changes in gene expression determined by RT-PCR. Secreted VEGF-C protein in conditioned media from RPE was examined by western blotting and ELISA analysis. The ability of VEGF-C to elicit tube formation in choroidal endothelial cells was assayed by an in vitro Matrigel model. Result: VEGF-A and glucose upregulated VEGF-C mRNA expression and increased the secretion of VEGF-C protein into the culture medium. VEGF-A, but not glucose alone, stimulated VEGFR-3 mRNA expression. VEGF-C acted synergistically with VEGF-A to promote in vitro tube formation by choroidal endothelial cells. Conclusion: VEGF-A has a critical role in the orchestration of VEGF-C expression in RPE cells and the synergistic action of VEGF-C with VEGF-A may play an important part in the aetiology of CNV.

Original languageEnglish (US)
Pages (from-to)1052-1059
Number of pages8
JournalBritish Journal of Ophthalmology
Volume90
Issue number8
DOIs
StatePublished - Aug 2006
Externally publishedYes

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Vascular Endothelial Growth Factor C
Retinal Pigments
Vascular Endothelial Growth Factor A
Epithelial Cells
Vascular Endothelial Growth Factor Receptor-3
Choroidal Neovascularization
Glucose
Endothelial Cells
Western Blotting
Enzyme-Linked Immunosorbent Assay
human VEGFC protein
Polymerase Chain Reaction
Messenger RNA
Conditioned Culture Medium
Culture Media
Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

VEGF-A regulates the expression of VEGF-C in human retinal pigment epithelial cells. / Zhao, B.; Ma, A.; Cai, J.; Boulton, Mike.

In: British Journal of Ophthalmology, Vol. 90, No. 8, 08.2006, p. 1052-1059.

Research output: Contribution to journalArticle

Zhao, B. ; Ma, A. ; Cai, J. ; Boulton, Mike. / VEGF-A regulates the expression of VEGF-C in human retinal pigment epithelial cells. In: British Journal of Ophthalmology. 2006 ; Vol. 90, No. 8. pp. 1052-1059.
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