VEGF distribution in proliferative diabetic retinopathy

D. Foreman, G. Williams, M. E. Boulton, D. McLeod

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Abstract

Purpose. To determine the staining pattern of VEGF at different stages of diabetic retinopathy. Method. Immunohistochemical localisation of VEGF, using an antibody raised against the 165 amino acid form of human VEGF, was carried out on specimens of normal human retina (n=15), diabetic retinas a) with no overt retinopathy (n=25) b) with active proliferative retinopathy (n=5), c) which had undergone successful photocoagulation therapy (n=12), and surgically resected PDR preretinal membranes (n=19). The degree and pattern of positive immunostaining was recorded. Results. VEGF staining was absent from the majority of normal retinas, with weak staining of endothelial cells of retinal vessels in 7/15 specimens. Immunostaining for VEGF was markedly increased in the majority of diabetic retinas with no overt retinopathy (20/25), especially in the thickened basement membranes of the retinal vessels (15/25). In all retinas with active neovascularisation immunostaining was intense, especially associated with intraretinal vessels adjacent to areas of active preretinal neovascularisation (5/5). Interestingly, VEGF-positive inner retinal vessels were found in less than half (5/12) of the diabetic retinas that had undergone successful laser therapy i.e. those with no active neovascularisation. Furthermore, VEGF staining was intense in all excised PDR membranes. Conclusions. This study supports a role for VEGF in the aetiology of diabetic retinopathy.

Original languageEnglish (US)
Pages (from-to)S973
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Foreman, D., Williams, G., Boulton, M. E., & McLeod, D. (1996). VEGF distribution in proliferative diabetic retinopathy. Investigative Ophthalmology and Visual Science, 37(3), S973.