Venous and arterial endothelial proteomics: Mining for markers and mechanisms of endothelial diversity

Matthew R. Richardson, Xianyin Lai, Frank Witzmann, Mervin Yoder

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Endothelial cells (ECs) line the inside of arterial and venous blood vessels in a continuous monolayer and have the important function of responding to environmental cues to regulate vascular tone and new blood vessel formation. They also have well-defined roles in supporting tumorigenesis, and alterations in their function lead to cardiovascular disease. Consequently, ECs have been studied extensively as a cellular model of both normal and abnormal physiology. Despite their importance and the increased utility of proteomic tools in medical research, there are relatively few publications on the topic of vascular endothelial proteomics. A thorough search of the literature mined 52 publications focused exclusively on arterial and/or venous endothelial proteomics. These studies mostly relied upon examination of whole-cell lysates from cultured human umbilical vein ECs to investigate in vitro effects of various molecules, such as VEGF in the context of altering human umbilical vein EC functions related to angiogenesis. Only a few of these publications focused solely on a proteomic characterization of ECs and our analysis further revealed a lack of published studies incorporating proteomic analysis of freshly isolated ECs from tissues or in vitro conditions that mimic in vivo variables, such as oxygen tension and shear stress. It is the purpose of this article to account for the diversity of vascular EC proteomic investigations and comment on the issues that have been and should be addressed in future work.

Original languageEnglish
Pages (from-to)823-831
Number of pages9
JournalExpert Review of Proteomics
Volume7
Issue number6
DOIs
StatePublished - Dec 2010

Fingerprint

Endothelial cells
Proteomics
Endothelial Cells
Blood Vessels
Publications
Human Umbilical Vein Endothelial Cells
Blood vessels
Physiology
Vascular Endothelial Growth Factor A
Endothelium
Cues
Biomedical Research
Cultured Cells
Carcinogenesis
Cardiovascular Diseases
Shear stress
Monolayers
Oxygen
Cell Line
Tissue

Keywords

  • artery
  • endothelial
  • human umbilical vein endothelial cell
  • HUVEC
  • LC-MS/MS
  • vein

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Venous and arterial endothelial proteomics : Mining for markers and mechanisms of endothelial diversity. / Richardson, Matthew R.; Lai, Xianyin; Witzmann, Frank; Yoder, Mervin.

In: Expert Review of Proteomics, Vol. 7, No. 6, 12.2010, p. 823-831.

Research output: Contribution to journalArticle

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