Vesicular stomatitis virus matrix protein impairs CD1d-mediated antigen presentation through activation of the p38 MAPK pathway

Gourapura J. Renukaradhya, Masood A. Khan, Daniel Shaji, Randy Brutkiewicz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Natural killer T (NKT) cells are unique T lymphocytes that recognize CD1d-bound lipid antigens and play an important role in both innate and acquired immune responses against infectious diseases and tumors. We have already shown that a vesicular stomatitis virus (VSV) infection results in the rapid inhibition of murine CD1d-mediated antigen presentation to NKT cells. In the present study, it was found that the VSV matrix (VSV-M) protein is an important element in this decrease in antigen presentation postinfection. The VSV-M protein altered the intracellular distribution of murine CD1d molecules, resulting in qualitative (but not quantitative) changes in cell surface CD1d expression. The M protein was distributed throughout the infected cell, and it was found to activate the mitogen-activated protein kinase (MAPK) p38 very early postinfection. Infection of CD1d+ cells with a temperature-sensitive VSV-M mutant at the nonpermissive temperature both substantially reversed the inhibition of antigen presentation by CD1d and delayed the activation of p38. Thus, the VSV-M protein plays an important role in permitting the virus to evade important components of the innate immune response by regulating specific MAPK pathways.

Original languageEnglish
Pages (from-to)12535-12542
Number of pages8
JournalJournal of Virology
Volume82
Issue number24
DOIs
StatePublished - Dec 2008

Fingerprint

CD1d Antigen
Vesiculovirus
antigen presentation
Vesicular Stomatitis
Antigen Presentation
p38 Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Natural Killer T-Cells
Viruses
Innate Immunity
T-lymphocytes
natural killer cells
Proteins
Temperature
proteins
Virus Diseases
Mitogen-Activated Protein Kinases
Communicable Diseases
T-Lymphocytes
mice

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Vesicular stomatitis virus matrix protein impairs CD1d-mediated antigen presentation through activation of the p38 MAPK pathway. / Renukaradhya, Gourapura J.; Khan, Masood A.; Shaji, Daniel; Brutkiewicz, Randy.

In: Journal of Virology, Vol. 82, No. 24, 12.2008, p. 12535-12542.

Research output: Contribution to journalArticle

Renukaradhya, Gourapura J. ; Khan, Masood A. ; Shaji, Daniel ; Brutkiewicz, Randy. / Vesicular stomatitis virus matrix protein impairs CD1d-mediated antigen presentation through activation of the p38 MAPK pathway. In: Journal of Virology. 2008 ; Vol. 82, No. 24. pp. 12535-12542.
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