Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms

Han Wei Wang, Ning Chai, Song Hu, Wei Dou, David Umulis, Lihong V. Wang, Michael Sturek, Robert Lucht, Ji Xin Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We realize vibrational photoacoustic microscopy (VPA) using molecular excitation of overtone vibration and acoustic detection of the resultant pressure transients. We demonstrate 3-D VPA imaging of lipid-rich atherosclerotic plaques and lipid bodies in living Drosophila larvae by exciting 2nd overtone of the CH bond stretch around 8300 cm-1. Depth-resolved spectral analysis and a penetration depth of several mm are available.

Original languageEnglish
Title of host publicationOptics InfoBase Conference Papers
StatePublished - 2011
EventCLEO: Applications and Technology, CLEO_AT 2011 - Baltimore, MD, United States
Duration: May 1 2011May 6 2011

Other

OtherCLEO: Applications and Technology, CLEO_AT 2011
CountryUnited States
CityBaltimore, MD
Period5/1/115/6/11

Fingerprint

Photoacoustic microscopy
photoacoustic microscopy
organisms
Lipids
lipids
Tissue
molecular excitation
sound detecting and ranging
transient pressures
larvae
Drosophila
harmonics
Imaging techniques
Spectrum analysis
Vibrations (mechanical)
spectrum analysis
penetration
Acoustics
methylidyne
vibration

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Wang, H. W., Chai, N., Hu, S., Dou, W., Umulis, D., Wang, L. V., ... Cheng, J. X. (2011). Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms. In Optics InfoBase Conference Papers

Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms. / Wang, Han Wei; Chai, Ning; Hu, Song; Dou, Wei; Umulis, David; Wang, Lihong V.; Sturek, Michael; Lucht, Robert; Cheng, Ji Xin.

Optics InfoBase Conference Papers. 2011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, HW, Chai, N, Hu, S, Dou, W, Umulis, D, Wang, LV, Sturek, M, Lucht, R & Cheng, JX 2011, Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms. in Optics InfoBase Conference Papers. CLEO: Applications and Technology, CLEO_AT 2011, Baltimore, MD, United States, 5/1/11.
Wang HW, Chai N, Hu S, Dou W, Umulis D, Wang LV et al. Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms. In Optics InfoBase Conference Papers. 2011
Wang, Han Wei ; Chai, Ning ; Hu, Song ; Dou, Wei ; Umulis, David ; Wang, Lihong V. ; Sturek, Michael ; Lucht, Robert ; Cheng, Ji Xin. / Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms. Optics InfoBase Conference Papers. 2011.
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