Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms

Han Wei Wang, Ning Chai, Song Hu, Wei Dou, David Umulis, Lihong V. Wang, Michael Sturek, Robert Lucht, Ji Xin Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We realize vibrational photoacoustic microscopy (VPA) using molecular excitation of overtone vibration and acoustic detection of the resultant pressure transients. We demonstrate 3-D VPA imaging of lipid-rich atherosclerotic plaques and lipid bodies in living Drosophila larvae by exciting 2nd overtone of the CH bond stretch around 8300 cm-1. Depth-resolved spectral analysis and a penetration depth of several mm are available.

Original languageEnglish (US)
Title of host publicationCLEO
Subtitle of host publicationApplications and Technology, CLEO_AT 2011
PublisherOptical Society of America (OSA)
ISBN (Print)9781557529107
DOIs
StatePublished - 2011
EventCLEO: Applications and Technology, CLEO_AT 2011 - Baltimore, MD, United States
Duration: May 1 2011May 6 2011

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceCLEO: Applications and Technology, CLEO_AT 2011
CountryUnited States
CityBaltimore, MD
Period5/1/115/6/11

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Wang, H. W., Chai, N., Hu, S., Dou, W., Umulis, D., Wang, L. V., Sturek, M., Lucht, R., & Cheng, J. X. (2011). Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms. In CLEO: Applications and Technology, CLEO_AT 2011 (Optics InfoBase Conference Papers). Optical Society of America (OSA). https://doi.org/10.1364/cleo_at.2011.jtug3