The use of noninvasive approaches such as fluorescence microscopy to detect events as they occur inside living cells is providing remarkable insight into molecular processes. Just in the last decade, the development of new genetically encoded probes, coupled with advances in digital image acquisition and analysis, has dramatically improved our ability to obtain quantitative measurements from living cells. Specifically, the cloning of the jellyfish Aequorea victoria green fluorescent protein (GFP; Prasher et al. 1992) sparked a revolution in studies of cell biology and physiology. For the first time, it became possible to produce genetically encoded fluorescent markers inside living cells and organisms (Chalfie et al. 1994; Inouye and Tsuji 1994). The utility of the fluorescent proteins (FPs) as noninvasive probes has been repeatedly proven by their integration into a variety of different living systems (reviewed by Hadjantonakis et al. 2003; Stewart 2006).
|Original language||English (US)|
|Title of host publication||Flim Microscopy in Biology and Medicine|
|Number of pages||25|
|State||Published - Jan 1 2009|
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Biochemistry, Genetics and Molecular Biology(all)