Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix

William H J Douglas, James A. McAteer, Robert T. Dell'orco, David Phelps

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A one-step vital stain is described for the macroscopic visualization of histotypic cell aggregates in fetal rat lung organotypic cultures. Organotypic cultures are incubated in 0.05-0.1% 2,3,5′-triphenyl tetrazolium chloride (TTC) in culture medium (37°C). Living cells reduce the tetrazole to a water-insoluble red colored formazan. Cell aggregates appear as densely stained foci against the lighter background of the Gelfoam substrate. Stained cultures may be scanned macroscopically to determine the degree of reaggregation and assess cell viability. Identification of aggregates by TTC staining improves the efficiency of tissue processing for electron microscopy and does not alter the ultrastructural appearance of the cultured cells.

Original languageEnglish (US)
Pages (from-to)306-312
Number of pages7
JournalIn Vitro
Volume16
Issue number4
DOIs
StatePublished - Apr 1980
Externally publishedYes

Fingerprint

Porifera
Collagen
Visualization
Cells
Chlorides
Absorbable Gelatin Sponge
Formazans
Electron microscopy
Culture Media
Rats
Cultured Cells
Cell Survival
Electron Microscopy
Coloring Agents
Tissue
Staining and Labeling
Lung
Water
Substrates
Processing

Keywords

  • fetal lung
  • Gelfoam
  • organotypic culture
  • tetrazolium chloride

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Douglas, W. H. J., McAteer, J. A., Dell'orco, R. T., & Phelps, D. (1980). Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix. In Vitro, 16(4), 306-312. https://doi.org/10.1007/BF02618336

Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix. / Douglas, William H J; McAteer, James A.; Dell'orco, Robert T.; Phelps, David.

In: In Vitro, Vol. 16, No. 4, 04.1980, p. 306-312.

Research output: Contribution to journalArticle

Douglas, WHJ, McAteer, JA, Dell'orco, RT & Phelps, D 1980, 'Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix', In Vitro, vol. 16, no. 4, pp. 306-312. https://doi.org/10.1007/BF02618336
Douglas, William H J ; McAteer, James A. ; Dell'orco, Robert T. ; Phelps, David. / Visualization of cellular aggregates cultured on a three dimensional collagen sponge matrix. In: In Vitro. 1980 ; Vol. 16, No. 4. pp. 306-312.
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