Volume regulation in cultured cells derived from human retinal pigment epithelium

Research output: Contribution to journalArticle

Abstract

To characterize volume regulatory mechanisms, unidirectional Rb+ efflux and influx, unidirectional Cl- influx, and cell volume were measured in cultured human retinal pigment epithelium (HRPE). The HRPE was found to be capable of both regulatory volume increase (RVI), in response to a hypertonic challenge, and regulatory volume decrease (RVD), in response to a hypotonic challenge. Bumetanide-sensitive Rb+ influx increased almost threefold on incubation in a hypertonic (390 mosmol/kgH2O) medium. Bumetanide-insensitive Rb+ influx was activated by hypotonic (190 mosmol/kgH2O) challenge as well as by treatment with N-ethylmaleimide (NEM). Exposure to hypotonic media also activated unidirectional Cl- influx and unidirectional Rb+ efflux. Both the RVD and hypotonically activated Rb+ efflux were inhibited by the K+- channel blocker barium. On the other hand, hypotonically activated Rb+ influx was increased by barium treatment. In sum, the HRPE exhibits volume- sensitive transport mechanisms over a range of volumes from 190 to 390 mosmol/kgH2O. Cultured HRPE possess hypertonically activated Na-K-Cl cotransport, hypotonically activated K-Cl cotransport, and a barium- inhibitable hypotonically activated K+ efflux pathway.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume266
Issue number3 35-3
StatePublished - 1994
Externally publishedYes

Fingerprint

Retinal Pigments
Retinal Pigment Epithelium
Cultured Cells
Barium
Cells
Bumetanide
Ethylmaleimide
Cell Size

Keywords

  • barium
  • epithelial transport
  • ion transport
  • N-ethylmaleimide
  • potassium chloride cotransport
  • regulatory volume decrease
  • regulatory volume increase
  • sodium potassium chloride cotransport

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

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title = "Volume regulation in cultured cells derived from human retinal pigment epithelium",
abstract = "To characterize volume regulatory mechanisms, unidirectional Rb+ efflux and influx, unidirectional Cl- influx, and cell volume were measured in cultured human retinal pigment epithelium (HRPE). The HRPE was found to be capable of both regulatory volume increase (RVI), in response to a hypertonic challenge, and regulatory volume decrease (RVD), in response to a hypotonic challenge. Bumetanide-sensitive Rb+ influx increased almost threefold on incubation in a hypertonic (390 mosmol/kgH2O) medium. Bumetanide-insensitive Rb+ influx was activated by hypotonic (190 mosmol/kgH2O) challenge as well as by treatment with N-ethylmaleimide (NEM). Exposure to hypotonic media also activated unidirectional Cl- influx and unidirectional Rb+ efflux. Both the RVD and hypotonically activated Rb+ efflux were inhibited by the K+- channel blocker barium. On the other hand, hypotonically activated Rb+ influx was increased by barium treatment. In sum, the HRPE exhibits volume- sensitive transport mechanisms over a range of volumes from 190 to 390 mosmol/kgH2O. Cultured HRPE possess hypertonically activated Na-K-Cl cotransport, hypotonically activated K-Cl cotransport, and a barium- inhibitable hypotonically activated K+ efflux pathway.",
keywords = "barium, epithelial transport, ion transport, N-ethylmaleimide, potassium chloride cotransport, regulatory volume decrease, regulatory volume increase, sodium potassium chloride cotransport",
author = "Brian Kennedy",
year = "1994",
language = "English (US)",
volume = "266",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
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T1 - Volume regulation in cultured cells derived from human retinal pigment epithelium

AU - Kennedy, Brian

PY - 1994

Y1 - 1994

N2 - To characterize volume regulatory mechanisms, unidirectional Rb+ efflux and influx, unidirectional Cl- influx, and cell volume were measured in cultured human retinal pigment epithelium (HRPE). The HRPE was found to be capable of both regulatory volume increase (RVI), in response to a hypertonic challenge, and regulatory volume decrease (RVD), in response to a hypotonic challenge. Bumetanide-sensitive Rb+ influx increased almost threefold on incubation in a hypertonic (390 mosmol/kgH2O) medium. Bumetanide-insensitive Rb+ influx was activated by hypotonic (190 mosmol/kgH2O) challenge as well as by treatment with N-ethylmaleimide (NEM). Exposure to hypotonic media also activated unidirectional Cl- influx and unidirectional Rb+ efflux. Both the RVD and hypotonically activated Rb+ efflux were inhibited by the K+- channel blocker barium. On the other hand, hypotonically activated Rb+ influx was increased by barium treatment. In sum, the HRPE exhibits volume- sensitive transport mechanisms over a range of volumes from 190 to 390 mosmol/kgH2O. Cultured HRPE possess hypertonically activated Na-K-Cl cotransport, hypotonically activated K-Cl cotransport, and a barium- inhibitable hypotonically activated K+ efflux pathway.

AB - To characterize volume regulatory mechanisms, unidirectional Rb+ efflux and influx, unidirectional Cl- influx, and cell volume were measured in cultured human retinal pigment epithelium (HRPE). The HRPE was found to be capable of both regulatory volume increase (RVI), in response to a hypertonic challenge, and regulatory volume decrease (RVD), in response to a hypotonic challenge. Bumetanide-sensitive Rb+ influx increased almost threefold on incubation in a hypertonic (390 mosmol/kgH2O) medium. Bumetanide-insensitive Rb+ influx was activated by hypotonic (190 mosmol/kgH2O) challenge as well as by treatment with N-ethylmaleimide (NEM). Exposure to hypotonic media also activated unidirectional Cl- influx and unidirectional Rb+ efflux. Both the RVD and hypotonically activated Rb+ efflux were inhibited by the K+- channel blocker barium. On the other hand, hypotonically activated Rb+ influx was increased by barium treatment. In sum, the HRPE exhibits volume- sensitive transport mechanisms over a range of volumes from 190 to 390 mosmol/kgH2O. Cultured HRPE possess hypertonically activated Na-K-Cl cotransport, hypotonically activated K-Cl cotransport, and a barium- inhibitable hypotonically activated K+ efflux pathway.

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KW - epithelial transport

KW - ion transport

KW - N-ethylmaleimide

KW - potassium chloride cotransport

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KW - regulatory volume increase

KW - sodium potassium chloride cotransport

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