Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells

R. E. Schwarz, D. C. Wojciechowicz, A. I. Picon, Margaret Schwarz, P. B. Paty

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Lectin binding specificities for carbohydrate allow phenotypic and functional characterization of membrane-associated glycoproteins expressed on cancer cells. This analysis examined wheatgerm agglutinin binding to pancreatic cancer cells in vitro and the resulting toxicity. Membrane preparations of nine human pancreatic carcinoma cell lines were studied for lectin binding using wheatgerm agglutinin (WGA), concanavalin A (ConA) and phytohaemagglutinin-L (PHA-L) in a lectin blot analysis. Cell proliferation in vitro was measured by thymidine incorporation in the absence or presence of lectins at various concentrations. Sialic acid binding lectins or succinyl-WGA (succWGA) served as controls. WGA toxicity was tested after swainsonine or neuraminidase pretreatment. Binding and uptake of fluorescein-labelled lectins was studied under fluorescence microscopy. All pancreatic cell lines displayed high WGA membrane binding, primarily to sialic acid residues. Other lectins were bound with weak to moderate intensity only. Lectin toxicity corresponded to membrane binding intensity, and was profound in case of WGA (ID50 at 2.5-5 μg ml-1). WGA exposure induced chromatin condensation, nuclear fragmentation and DNA release consistent with apoptosis. Important steps for WGA toxicity included binding to sialic acid on swainsonine-sensitive carbohydrate and lectin internalization. There was rapid cellular uptake and subsequent nuclear relocalization of WGA. In contradistinction to the other lectins studied, WGA proved highly toxic to human pancreatic carcinoma cells in vitro. WGA binding to sialic acid residues of N-linked carbohydrate, cellular uptake and subsequent affinity to N-acetyl glucosamine appear to be necessary steps. Further analysis of this mechanism of profound toxicity may provide insight relevant to the treatment of pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)1754-1762
Number of pages9
JournalBritish Journal of Cancer
Volume80
Issue number11
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Agglutinins
Pancreatic Neoplasms
Lectins
N-Acetylneuraminic Acid
Swainsonine
Carbohydrates
Membranes
Cell Line
Poisons
Glucosamine
Membrane Glycoproteins
Neuraminidase
Phytohemagglutinins
DNA Fragmentation
Concanavalin A
Fluorescein
Fluorescence Microscopy
Thymidine
Chromatin
Cell Proliferation

Keywords

  • Apoptosis
  • Lectin
  • Pancreatic cancer
  • Sialic acid surface binding
  • Swainsonine
  • Wheatgerm agglutinin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Schwarz, R. E., Wojciechowicz, D. C., Picon, A. I., Schwarz, M., & Paty, P. B. (1999). Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells. British Journal of Cancer, 80(11), 1754-1762. https://doi.org/10.1038/sj.bjc.6690593

Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells. / Schwarz, R. E.; Wojciechowicz, D. C.; Picon, A. I.; Schwarz, Margaret; Paty, P. B.

In: British Journal of Cancer, Vol. 80, No. 11, 1999, p. 1754-1762.

Research output: Contribution to journalArticle

Schwarz, RE, Wojciechowicz, DC, Picon, AI, Schwarz, M & Paty, PB 1999, 'Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells', British Journal of Cancer, vol. 80, no. 11, pp. 1754-1762. https://doi.org/10.1038/sj.bjc.6690593
Schwarz, R. E. ; Wojciechowicz, D. C. ; Picon, A. I. ; Schwarz, Margaret ; Paty, P. B. / Wheatgerm agglutinin-mediated toxicity in pancreatic cancer cells. In: British Journal of Cancer. 1999 ; Vol. 80, No. 11. pp. 1754-1762.
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