Whole-cell patch-clamp recordings on spinal cord slices

Ping Deng, Zao C. Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Whole-cell patch-clamp recording technique is a powerful tool to study intrinsic membrane properties and synaptic interactions in the spinal cord. Spinal cord slice is an idea preparation for electrophysiological studies under physiological and pharmacological manipulation that is difficult to perform in an in vivo preparation. Depending on experimental purposes, the extracellular and intracellular environment of neurons can be easily controlled during whole-cell recording to isolate membrane conductance of interest and to manipulate its modulation, which is important for addressing cellular mechanisms under particular physiological and pathological conditions. Several methods for preparing spinal cord slices have been developed for whole-cell patch-clamp recordings. Here we describe practical procedures for preparing spinal cord slices from adult rats and whole-cell recording from neurons in the spinal dorsal horn.

Original languageEnglish (US)
Title of host publicationPain Research
Subtitle of host publicationMethods and Protocols
EditorsDavid Luo
Number of pages8
StatePublished - Mar 16 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Current-clamp
  • Dorsal horn
  • Neuronal excitability
  • Spinal cord
  • Synaptic transmission
  • Voltage-clamp

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Deng, P., & Xu, Z. C. (2012). Whole-cell patch-clamp recordings on spinal cord slices. In D. Luo (Ed.), Pain Research: Methods and Protocols (pp. 65-72). (Methods in Molecular Biology; Vol. 851). https://doi.org/10.1007/978-1-61779-561-9_4