Widespread regional myocardial transfection by plasmid encoding Del-1 following retrograde coronary venous delivery

Dongming Hou, Fiona Maclaughlin, Mary Thiesse, Vipul R. Panchal, Bas C A M Bekkers, Elizabeth A. Wilson, Pamela I. Rogers, Michael C. Coleman, Keith L. March

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

This study quantifies myocardial transfection following percutaneous retrograde coronary venous delivery (RCVD) of a plasmid encoding human Del-1. RCVD of Del-1, GFP plasmid, or marker dye was conducted in 14 pigs. After selective cannulation of a coronary vein, a delivery site was confirmed by contrast injection and myocardial blush. Ten milliliters of plasmid hDel-1 or GFP was administered. Animals were euthanized 3 and 7 days post-RCVD. hDel-1 gene expression was evaluated by quantitative RT-PCR. An average myocardial expression of 4.5 × 105 copies hDel-1/μg total RNA was observed within the approximately 5 × 5 cm2 target tissue of the left ventricle. GFP expression was detected by fluorescent microscopy. hDel-1 protein expression was confirmed by immunohistochemistry. Regionalized myocardial expression was found in all pigs. hDel-1 RNA was not found in distant tissues except in the three pigs with prominent venovenous washout (PVW). These levels were 3 to 4 log unites lower than those found in myocardium. Single retrograde coronary venous administration resulted in efficient regional myocyte transfection of hDel-1 and GFP. This method may be useful and clinically feasible for myocardial angiogenesis therapy.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalCatheterization and Cardiovascular Interventions
Volume58
Issue number2
DOIs
StatePublished - Feb 1 2003

Fingerprint

Transfection
Plasmids
Swine
RNA
Catheterization
Muscle Cells
Heart Ventricles
Microscopy
Coronary Vessels
Myocardium
Coloring Agents
Immunohistochemistry
Gene Expression
Polymerase Chain Reaction
Injections
Proteins
Therapeutics

Keywords

  • Drug administration
  • Gene therapy
  • Myocardium

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Hou, D., Maclaughlin, F., Thiesse, M., Panchal, V. R., Bekkers, B. C. A. M., Wilson, E. A., ... March, K. L. (2003). Widespread regional myocardial transfection by plasmid encoding Del-1 following retrograde coronary venous delivery. Catheterization and Cardiovascular Interventions, 58(2), 207-211. https://doi.org/10.1002/ccd.10417

Widespread regional myocardial transfection by plasmid encoding Del-1 following retrograde coronary venous delivery. / Hou, Dongming; Maclaughlin, Fiona; Thiesse, Mary; Panchal, Vipul R.; Bekkers, Bas C A M; Wilson, Elizabeth A.; Rogers, Pamela I.; Coleman, Michael C.; March, Keith L.

In: Catheterization and Cardiovascular Interventions, Vol. 58, No. 2, 01.02.2003, p. 207-211.

Research output: Contribution to journalArticle

Hou, D, Maclaughlin, F, Thiesse, M, Panchal, VR, Bekkers, BCAM, Wilson, EA, Rogers, PI, Coleman, MC & March, KL 2003, 'Widespread regional myocardial transfection by plasmid encoding Del-1 following retrograde coronary venous delivery', Catheterization and Cardiovascular Interventions, vol. 58, no. 2, pp. 207-211. https://doi.org/10.1002/ccd.10417
Hou, Dongming ; Maclaughlin, Fiona ; Thiesse, Mary ; Panchal, Vipul R. ; Bekkers, Bas C A M ; Wilson, Elizabeth A. ; Rogers, Pamela I. ; Coleman, Michael C. ; March, Keith L. / Widespread regional myocardial transfection by plasmid encoding Del-1 following retrograde coronary venous delivery. In: Catheterization and Cardiovascular Interventions. 2003 ; Vol. 58, No. 2. pp. 207-211.
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