X inactivation phenotype in carriers of Pelizaeus-Merzbacher disease: Skewed in carriers of a duplication and random in carriers of point mutations

Karen Woodward, Karen Kirtland, Stephen Dlouhy, Wendy Raskind, Thomas Bird, Sue Malcolm, Dvorah Abeliovich

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Pelizaeus-Merzbacher disease (PMD) is an X-linked recessive disease caused by coding sequence mutations in the PLP gene, sub-microscopic duplications of variable sizes including the PLP gene or very rarely deletions of the PLP gene. We analysed the X inactivation pattern in blood of PMD female carriers with duplications and with point mutations. In the majority of duplication carriers (7/11), the X chromosome bearing the duplication was preferentially inactivated, whereas a random pattern of X inactivation was detected in point mutation carriers (3/3), a deletion carrier (1/1), affected females (4/4) who did not have a recognised mutation and normal control females. However 2/5 non-carrier female relatives of patients with a duplication, had skewed X inactivation. The skewed pattern of inactivation observed in most duplication carriers and not in mutation carriers suggests a) that there is selection against those cells in which the duplicated X chromosome is active and b) other expressed sequences within the duplicated region rather than mutant PLP may be responsible. Since the skewed X inactivation did not segregate with the disease in two families and the pattern of X inactivation was variable among the duplication carriers, the pattern X inactivation is an unsuitable diagnostic tool for female carriers of PMD.

Original languageEnglish (US)
Pages (from-to)449-454
Number of pages6
JournalEuropean Journal of Human Genetics
Volume8
Issue number6
DOIs
StatePublished - Jun 2000

Keywords

  • Deletion
  • Duplication
  • Pelizaeus-Merzbacher disease
  • PLP
  • PMD
  • X inactivation

ASJC Scopus subject areas

  • Genetics(clinical)

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