X-ray structure of human β3β3 alcohol dehydrogenase. The contribution of ionic interactions to coenzyme binding

Gerard J. Davis, William F. Bosron, Carol L. Stone, Kwabena Owusu-Dekyi, Thomas Hurley

Research output: Contribution to journalArticle

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Abstract

The three-dimensional structure of the human β3β3 dimeric alcohol dehydrogenase (β3) was determined to 2.4-Å resolution. β3 was crystallized as a ternary complex with the coenzyme NAD+ and the competitive inhibitor 4-iodopyrazole. β3 is a polymorphic variant at ADH2 that differs from β1 by a single amino acid substitution of Arg-369 → Cys. The available x-ray structures of mammalian alcohol dehydrogenases show that the side chain of Arg-369 forms an ion pair with the NAD(H) pyrophosphate to stabilize the E·NAD(H) complex. The Cys-369 side chain of β3 cannot form this interaction. The three-dimensional structures of β3 and β1 are virtually identical, with the exception that Cys-369 and two water molecules in β3 occupy the position of Arg-369 in β1. The two waters occupy the same positions as two guanidino nitrogens of Arg-369. Hence, the number of hydrogen bonding interactions between the enzyme and NAD(H) are the same for both isoenzymes. However, β3 differs from β1 by the loss of the electrostatic interaction between the NAD(H) pyrophosphate and the Arg-369 side chain. The equilibrium dissociation constants of β3 for NAD+ and NADH are 350-fold and 4000-fold higher, respectively, than those for β1. These changes correspond to binding free energy differences of 3.5 kcal/mol for NAD+ and 4.9 kcal/mol for NADH. Thus, the Arg-369 → Cys substitution of β3 isoenzyme destabilizes the interaction between coenzyme and β3 alcohol dehydrogenase.

Original languageEnglish
Pages (from-to)17057-17061
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number29
DOIs
StatePublished - 1996

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Alcohol Dehydrogenase
Coenzymes
NAD
X-Rays
X rays
Isoenzymes
Substitution reactions
Water
Amino Acid Substitution
Hydrogen Bonding
Coulomb interactions
Static Electricity
Free energy
Hydrogen bonds
Nitrogen
Ions
Amino Acids
Molecules
Enzymes

ASJC Scopus subject areas

  • Biochemistry

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X-ray structure of human β3β3 alcohol dehydrogenase. The contribution of ionic interactions to coenzyme binding. / Davis, Gerard J.; Bosron, William F.; Stone, Carol L.; Owusu-Dekyi, Kwabena; Hurley, Thomas.

In: Journal of Biological Chemistry, Vol. 271, No. 29, 1996, p. 17057-17061.

Research output: Contribution to journalArticle

Davis, Gerard J. ; Bosron, William F. ; Stone, Carol L. ; Owusu-Dekyi, Kwabena ; Hurley, Thomas. / X-ray structure of human β3β3 alcohol dehydrogenase. The contribution of ionic interactions to coenzyme binding. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 29. pp. 17057-17061.
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