XKCM1: A Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly

Claire Walczak, Timothy J. Mitchison, Arshad Desai

Research output: Contribution to journalArticle

439 Citations (Scopus)

Abstract

We isolated a cDNA clone encoding a kinesin-related protein, which we named XKCM1. Antibodies to XKCM1 stain mitotic centromeres and spindle poles. Immunodepletion and antibody addition experiments in an in vitro spindle assembly assay show that XKCM1 is required for both establishment and maintenance of mitotic spindles. The structures that form in the absence of XKCM1 contain abnormally long microtubules. This long microtubule defect can be rescued by the addition of purified XKCM1 protein. Analysis of microtubule dynamics in a clarified mitotic extract reveals that loss of XKCM1 function causes a 4-fold suppression in the catastrophe frequency. XKCM1 thus exhibits a novel activity for a kinesin-related protein by promoting microtubule depolymerization during mitotic spindle assembly.

Original languageEnglish (US)
Pages (from-to)37-47
Number of pages11
JournalCell
Volume84
Issue number1
DOIs
StatePublished - Jan 12 1996
Externally publishedYes

Fingerprint

Microtubule Proteins
Kinesin
Spindle Apparatus
Xenopus
Microtubules
Spindle Poles
Depolymerization
Proteins
Antibodies
Centromere
Poles
Assays
Coloring Agents
Complementary DNA
Clone Cells
Maintenance
Defects
Experiments

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

XKCM1 : A Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. / Walczak, Claire; Mitchison, Timothy J.; Desai, Arshad.

In: Cell, Vol. 84, No. 1, 12.01.1996, p. 37-47.

Research output: Contribution to journalArticle

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