Yeast model identifies ENTPD6 as a potential non-obstructive azoospermia pathogenic gene

Qian Wang, Chao Liu, Chaoming Tang, Huiping Guo, Yujiao Liu, Lina Wang, Haichao Zhao, Yongliang Shang, Yang Wen, Yuan Lin, Tao Zhou, Zuomin Zhou, Wen Dong, Zhibin Hu, Xuejiang Guo, Jiahao Sha, Wei Li

Research output: Contribution to journalArticle

Abstract

Approximately ten percent of male infertility is caused by non-obstructive azoospermia (NOA), but the etiologies of many NOA remain elusive. Recently, a genome-wide association study (GWAS) of NOA in Han Chinese men was conducted, and only a few genetic variants associated with NOA were found, which might have resulted from genetic heterogeneity. However, those variants that lack genome-wide significance might still be essential for fertility. Functional analysis of genes surrounding these variants in Drosophila identified some spermatogenesis-essential genes. As a complementary method of Drosophila screening, SK1 background Saccharomvces cerevisiae was used as a model to screen meiosis-related genes from the NOA GWAS data in this study. After functional screening, GDA1 (orthologous to human ENTPD6) was found to be a novel meiosis-related gene. The deletion of GDA1 resulted in the failure of yeast sporulation. Further investigations showed that Gda1p was important for pre-meiotic S phase entry. Interestingly, the meiotic role of Gda1p was dependent on its guanosine diphosphatase activity, but not it's cytoplasmic, transmembrane or stem domains. These yeast data suggest that ENTPD6 may be a novel meiosis-associated NOA-related gene, and the yeast model provides a good approach to analyze GWAS results of NOA.

Original languageEnglish (US)
Article number11762
JournalScientific Reports
Volume5
DOIs
StatePublished - Jul 8 2015
Externally publishedYes

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Azoospermia
Yeasts
Genome-Wide Association Study
Meiosis
Genes
Drosophila
Genetic Heterogeneity
Male Infertility
Essential Genes
Spermatogenesis
S Phase
Fertility
Genome

ASJC Scopus subject areas

  • General

Cite this

Yeast model identifies ENTPD6 as a potential non-obstructive azoospermia pathogenic gene. / Wang, Qian; Liu, Chao; Tang, Chaoming; Guo, Huiping; Liu, Yujiao; Wang, Lina; Zhao, Haichao; Shang, Yongliang; Wen, Yang; Lin, Yuan; Zhou, Tao; Zhou, Zuomin; Dong, Wen; Hu, Zhibin; Guo, Xuejiang; Sha, Jiahao; Li, Wei.

In: Scientific Reports, Vol. 5, 11762, 08.07.2015.

Research output: Contribution to journalArticle

Wang, Q, Liu, C, Tang, C, Guo, H, Liu, Y, Wang, L, Zhao, H, Shang, Y, Wen, Y, Lin, Y, Zhou, T, Zhou, Z, Dong, W, Hu, Z, Guo, X, Sha, J & Li, W 2015, 'Yeast model identifies ENTPD6 as a potential non-obstructive azoospermia pathogenic gene', Scientific Reports, vol. 5, 11762. https://doi.org/10.1038/srep11762
Wang, Qian ; Liu, Chao ; Tang, Chaoming ; Guo, Huiping ; Liu, Yujiao ; Wang, Lina ; Zhao, Haichao ; Shang, Yongliang ; Wen, Yang ; Lin, Yuan ; Zhou, Tao ; Zhou, Zuomin ; Dong, Wen ; Hu, Zhibin ; Guo, Xuejiang ; Sha, Jiahao ; Li, Wei. / Yeast model identifies ENTPD6 as a potential non-obstructive azoospermia pathogenic gene. In: Scientific Reports. 2015 ; Vol. 5.
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